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Calcium transport in isolated bone cells I. Bone cell isolation procedures
Author(s) -
Dziak Rosemary,
Brand John S.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040840109
Subject(s) - calcium , collagenase , calvaria , chemistry , hyaluronidase , filtration (mathematics) , extracellular , digestion (alchemy) , chromatography , biochemistry , enzyme , statistics , mathematics , organic chemistry , in vitro
Abstract Bone cells were isolated by collagenase‐hyaluronidase digestion of 19–20 day‐old fetal rat calvaria. It is shown that contamination from calcified matrix can give erroneously high values for total cell calcium and can mask intracellular calcium exchange. Filtration of the cell suspension through 35 μ mesh nylon net and a five minute treatment with a cold pH 6.0 isotonic salt solution removed the contamination. Total calcium for cells prepared in this manner was 16.8 mmoles/kg wet weight. 45 Ca uptake studies revealed an active component of calcium exchange.