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Potassium content of single human red cells measured with an electron probe
Author(s) -
Gary Kirk R.,
Crenshaw Miles A.,
Tosteson D. C.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040840104
Subject(s) - potassium , beryllium , chemistry , absorption (acoustics) , analytical chemistry (journal) , materials science , biophysics , chromatography , biology , organic chemistry , composite material
The potassium content of single human red cells was measured with an electron probe. Cells were placed on beryllium discs and coated with a thin layer of dibutyl pthalate to prevent loss of cellular contents. Samples were stable under the electron beam during analysis for more than 15 minutes and could be stored for long periods of time. Primary standards were prepared by loading red cells with varying known amounts of potassium in order to circumvent the corrections for absorption. The X‐ray intensity was found to be directly proportional to the potassium content of the cells.