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Neoplastic differentiation: Characteristics of cell lines derived from a murine teratocarcinoma
Author(s) -
Lehman J. M.,
Speers W. C.,
Swartzendruber D. E.,
Pierce G. B.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040840103
Subject(s) - teratocarcinoma , biology , embryonal carcinoma , yolk sac , cell culture , neuroepithelial cell , cellular differentiation , mesenchymal stem cell , stem cell , microbiology and biotechnology , pathology , cancer research , embryo , genetics , medicine , neural stem cell , gene
Abstract Monolayer cultures of a mouse teratocarcinoma were established in vitro. These cultures contained embryonal carcinoma, the malignant stem cell, and its differentiated progeny: parietal yolk sac, neuroepithelial, and mesenchymal cells. Tissues such as squamous epithelium, cartilage, striated muscle, neuroepithelium, and glands were produced from embryonal carcinoma that was maintained under conditions of long term culture. Frequent subcultivation with pancreatin allowed the establishment of cell lines of embryonal carcinoma which have been maintained for more than 18 months in vitro and continue to produce differentiated cells under specific culture conditions. Chromosomally these lines of embryonal carcinoma have a stem line of 39 chromosomes. Two lines of parietal yolk sac cells have been established which produce basement membrane, are not tumorigenic, and chromosomally are hypotetraploid. This system may yield information concerning neoplastic differentiation and its possible use in therapy for cancer.