Premium
Peritoneal exudate cells. I. Growth requirement of cells capable of forming colonies in soft agar
Author(s) -
Lin HsiuSan,
Stewart Carleton C.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040830307
Subject(s) - exudate , peritoneal cavity , bone marrow , biology , in vitro , agar , peripheral blood mononuclear cell , plating efficiency , microbiology and biotechnology , immunology , anatomy , biochemistry , bacteria , botany , genetics
Mouse peritoneal exudate cells induced by thioglycollate medium can form colonies in soft agar with a plating efficiency of about 5% (0.6%–10%). Cells from an unstimulated peritoneal cavity form no colonies or have a plating efficiency of less than 0.001 %. These colony‐forming cells from the peritoneal exudate are similar to bone marrow colony‐forming cells in vitro in that they both require a substance(s) present in conditioned medium from L‐cells or mouse embryo fibroblasts or the serum from endotoxin‐treated mice for the initiation and the continuation of their growth. However, peritoneal exudate colony‐forming cells have a much longer initial lag period (10–14 days) and can survive longer in the absence of L‐cell conditioned medium than bone marrow colony‐forming cells. Only mononuclear cells, presumably macrophages, are observed in peritoneal exudate colonies, whereas bone marrow cell colonies contain both polymorphonuclear cells and macrophages.