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Effect of growth conditions on the activity of ornithine decarboxylase in cultured hepatoma cells. II. Effect of serum and insulin
Author(s) -
Hogan Brigid L. M.,
McIlhinney Ann,
Murden Susan
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040830305
Subject(s) - ornithine decarboxylase , medicine , insulin , endocrinology , stimulation , tyrosine aminotransferase , transaminase , chemistry , basal (medicine) , dna synthesis , polyamine , ornithine , enzyme , biology , biochemistry , enzyme inducer , in vitro , arginine , amino acid
HTC cells incubated in the absence of serum for more than 14 hours have very low levels of ornithine decarboxylase, the first enzyme on the pathway of polyamine synthesis. Readdition of serum causes an increase in the activity of ODC, reaching a maximum on average 17 times above the basal level after five hours. This increase is due in part to a decrease in the apparent rate of degradation of ODC, and also to a stimulation of its synthesis. Within the first two hours the serum induction of ODC is resistant to Actinomycin D. Insulin at 5 μm/ml alsocauses an increase in ODC activity but only after a delay of two hours, in contrast to its more rapid stimulation of tyrosine transaminase activity.