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Conditional lethal mutants of Chinese hamster cells: Mutants requiring exogenous carbondioxide for growth
Author(s) -
Scheffler I. E.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040830208
Subject(s) - chinese hamster , hypoxanthine guanine phosphoribosyltransferase , hypoxanthine , mutant , uridine , biochemistry , acetoin , chemistry , reversion , bicarbonate , biology , fermentation , dna , rna , organic chemistry , phenotype , gene , enzyme
Abstract A series of Chinese hamster cell lines were tested and found to be able to proliferate in the absence of added bicarbonate and carbondioxide if hypoxanthine and uridine were present in the medium. Conversely, cells incapable of salvaging one of these precursors, such as hypoxanthine‐guanine phosphoribosyltransferase (HGPRT − ) deficient cells did not multiply under these conditions. We describe another variant capable of utilizing hypoxanthine and uridine which has an absolute requirement for exogenous CO 2 /NaHCO 3 for growth. These cells appear to be defective in the complete oxidation of pyruvate to carbondioxide, and indications are that the entry of pyruvate into the Krebs cycle is affected.