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Effect of cAMP on nucleoside metabolism. I. Effect on thymidine transport and incorporation in monkey cells (CV‐1)
Author(s) -
Roller Barbra,
Hirai Kanji,
Defendi Vittorio
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040830202
Subject(s) - deoxyribonucleotide , thymidine , nucleoside , dna synthesis , nucleotide , dna , endogeny , biochemistry , cell culture , metabolism , deoxyribonucleoside , dna replication , biology , nucleotide salvage , microbiology and biotechnology , chemistry , genetics , oligonucleotide , gene
Abstract The effect of cAMP on a monkey kidney cell line (CV‐1) noninfected and infected by SV40 was studied. No effect was found on either growth rate or cell morphology when concentrations up to 1 m M of 3′5′ cAMP were used. However, cAMP was found to increase the incorporation of 3 H‐thymidine into both cellular and viral DNA without a net increase in DNA synthesis. This increased incorporation was found to be related to an enhanced uptake of thymidine into the nucleotide pool which is reflected in an increase of phosphorylated nucleotides. This, coupled with a lack of effect of cAMP on endogenous deoxyribonucleotide production, produces an increased specific activity of the deoxyribonucleotide triphosphates, with a resultant increase in specific activity of DNA.