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Selection for temperature‐sensitive mutants of diploid and tetraploid mammalian cells
Author(s) -
McBurney Michael W.,
Whitmore Gordon F.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040830110
Subject(s) - ploidy , chinese hamster ovary cell , mutant , colcemid , biology , genetics , selection (genetic algorithm) , cell culture , thymidine , microbiology and biotechnology , phenotype , gene , in vitro , artificial intelligence , computer science
Mammalian cell populations may be enriched for temperature‐sensitive ( ts ) mutants by the tritiated thymidine ( 3 H‐TdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the near‐diploid Chinese hamster ovary (CHO) cell line and on a line made “tetraploid” by Colcemid treatment. Clones of ts mutants were obtained from the diploid line, but in spite of repeated attempts, no ts mutants were isolated from the tetroploid line. The phenotypes of the new diploid ts mutants recovered were consistent with the particular selection regime employed.