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Induction of lysosomal enzymes in contact inhibited 3T3 cells
Author(s) -
Horvat A.,
Ács G.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040830109
Subject(s) - enzyme , cycloheximide , polymerase , biology , biochemistry , microbiology and biotechnology , dna polymerase , 3t3 cells , gene , transfection , protein biosynthesis
The pattern of changing activities of three lysosomal enzymes, N‐acetyl‐β‐D‐glucosaminidase, aryl sulfatase, and DNase II, and that of DNA polymerase was followed in homogenates of 3T3 cells during the logarithmic phase of growth and in stationary cultures. The change in activities of the polymerase and the lysosomal enzymes is antiparallel. DNA polymerase exhibits highest activity in growing cultures, and shows a three‐fold decline of the specific activity in stationary cultures. The lysosomal enzymes show a very marked increase in their specific activity after density saturation is reached, which can be prevented by the addition of cycloheximide. Colcemid added to logarithmically growing cultures also causes an increase in the specific activities of lysosomal enzymes.