Premium
Influence of the explantation milieu on intranuclear [Na], [K] and [Mg] of Chironomus thummi salivary gland cells
Author(s) -
Kroeger Heinrich,
Trösch Walter
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040830104
Subject(s) - salivary gland , sodium , chemistry , potassium , function (biology) , chironomus , biophysics , biology , biochemistry , botany , chironomidae , organic chemistry , larva , evolutionary biology
Intranuclear Na, K and Mg concentrations were determined in cells of salivary glands incubated for 1 h in selected NaCl/KCl/MgCl 2 media. By variation of the external milieu beyond “physiological” limits the intranuclear electrolytes can be shifted between ca 100 and 280 mM [K] i , between ca 8 and 100 mM [Na] i and between ca 5 and 75 mM [Mg] i . No significant competition or interactions of the 3 ionic species are apparent. The relationships [K] e : [K] i and [Na] e : [Na] i can best be described by a positive and linear, that between [Mg] e : [Mg] i by a negative and exponential function. Regression parameters are given which permit a computation of intranuclear [Na], [K] and [Mg] as induced by NaCl/KCl/MgCl 2 in any binary or triple combination that is tolerated by the explanted gland without visible damage.