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Isolation and characterization of revertant cell lines. IV. Direct selection of serum‐revertant sublines of SV40‐transformed 3T3 mouse cells
Author(s) -
Vogel A.,
Pollack R.
Publication year - 1973
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040820207
Subject(s) - biology , 3t3 cells , cell culture , fibroblast , microbiology and biotechnology , cell , isolation (microbiology) , phenotype , genetics , transfection , gene
SV101, the SV40‐transformed subline of the mouse fibroblast line 3T3, is both serum‐ and density transformed, since it grows in both 1% and 10% calf serum, and grows beyond confluence in 10% calf serum. Negative selection at low cell density in 1% calf serum or in 10% agamma‐depleted serum permits direct recovery of serum‐revertant sublines of SV101. These sublines are unable to grow in 1% calf serum. Although negative selection at high cell density in 10% calf serum is known to permit recovery of density‐revertant sublines of SV101, most density‐revertants are not serum‐revertant. However, all serum‐revertants isolated so far are density‐revertant as well.