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Proteins from melanosomes of mouse and chick pigment cells
Author(s) -
Doezema Philip
Publication year - 1973
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040820108
Subject(s) - melanosome , sodium dodecyl sulfate , acrylamide , pigment , gel electrophoresis , sodium hydroxide , electrophoresis , chemistry , polyacrylamide gel electrophoresis , sodium , biochemistry , melanin , biology , microbiology and biotechnology , enzyme , monomer , organic chemistry , polymer
Purified subcellular fractions containing melanosomes from B‐16 mouse melanoma were treated with 2% sodium dodecyl sulfate or 0.5 M sodium hydroxide to dissolve protein. Quantitative measurements indicate that each melanosome contains 0.065×10 −10 of protein or about 19% by weight. SDS acrylamide gel electrophoresis of proteins from purified melanosomes resolved six polypeptide bands of major density and about 15 minor bands. These results indicate that the melanosome may be more complex than previous genetic, biochemical or morphological evidence had suggested.