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Characteristics of calcium accumulation by lymphocytes and alterations in the process induced by phytohemagglutinin
Author(s) -
Whitney Richard B.,
Sutherland Robert M.
Publication year - 1973
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040820103
Subject(s) - calcium , cycloheximide , efflux , magnesium , chemistry , diffusion , medicine , biophysics , biochemistry , endocrinology , biology , protein biosynthesis , physics , organic chemistry , thermodynamics
Calcium uptake by normal human lymphocytes was found to be a saturable process which was competitively inhibited by manganese indicating the existence of a carrier‐mediated mechanism for calcium uptake. Exchange diffusion was not observed, Phytohemagglutinin (PHA) significantly stimulated calcium uptake within minutes after treatment. The increased uptake was attributed to a decreased K m for the proposed membrane carrier rather than to an increased V max . Also PHA did not stimulate a normally unused exchange diffusion process, nor did it affect calcium efflux. Uptake by both unstimulated and PHA‐treated lymphocytes was not influenced by magnesium or by cycloheximide or actinomycin D.