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Enhancement by purine nucleosides and nucleotides of serum‐induced DNA synthesis in quiescent 3T3 cells
Author(s) -
Schor Seth,
Rozengurt Enrique
Publication year - 1973
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040810306
Subject(s) - hypoxanthine , purine , nucleotide , guanine , dna , dna synthesis , chemistry , purine metabolism , pyrimidine , biochemistry , microbiology and biotechnology , biology , enzyme , gene
Purine nucleotides and nucleosides were found to enhance the DNA synthesis in quiescent 3T3 cells induced either by fresh medium containing calf serum or by the addition of the β‐globulin serum fraction to depleted medium. A two to threefold enhancement has been obtained under these conditions. The adenine and hypoxanthine based compounds were more effective than the guanine ones in the concentration range tested (10 −5 −−10 −3 M), while the pyrimidine compounds were without effect. The degree of enhancement obtained with the purine compounds depended on the type and amount of serum used to stimulate DNA synthesis. A maximal degree of enhancement was obtained when the purine compounds were present for only the first three hours after the addition of serum and thus 18–20 hours before the actual onset of DNA synthesis.