Premium
The relation between cell division rate and 3 HTdR incorporation in organ culture
Author(s) -
Simnett John D.,
Fisher Jean M.
Publication year - 1973
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040810206
Subject(s) - colcemid , mitotic index , dna synthesis , thymidine , mitosis , biology , intracellular , cell division , endogeny , andrology , organ culture , cell culture , metaphase , labelling , dna , cell , microbiology and biotechnology , endocrinology , biochemistry , genetics , chromosome , in vitro , medicine , gene
Abstract Rates of incorporation of labelled thymidine (RIT), radioautographic labelling index for DNA synthesis (LI) and mitotic incidence following colcemid metaphase arrest (MI) were measured in organ cultures of newborn and adult rat lung. In adult cultures these three parameters correlated well, being low after explantation and reaching a maximum after two to three days. In newborn cultures RIT fell several fold over the first 24 hours after explantation and, in this respect, did not correlate with LI and MI. The changes in RIT over the first 24 hours appear to be due to changes in the degree of competition between endogenous TdR and exogenous labelled TdR, probably caused by leakage of the intracellular thymidine pool following explantation. The report emphasizes the need to check RIT data against radioautographic evidence before accepting it as an index of DNA synthesis.