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Azaguanine resistant hamster cell lines not deficient in hypoxanthine‐guanine phosphoribosyl transferase
Author(s) -
Morrow John,
Colofiore Joseph,
Rintoul David
Publication year - 1973
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040810112
Subject(s) - hypoxanthine , guanine , mutant , chinese hamster , hypoxanthine guanine phosphoribosyltransferase , biology , cell culture , hamster , microbiology and biotechnology , population , genetics , biochemistry , nucleotide , enzyme , medicine , gene , environmental health
Using a serial selection technique in which Chinese hamster cells were treated first with 8‐azaguanine and then subsequently with HAT medium it was found that approximately 15% of azaguanine resistant clones were also resistant to HAT. Several such clones were subcultured and found to be stably resistant to azaguanine, in some cases at a higher level than the usual azaguanine resistant mutants which are HAT sensitive. Measurements of hypoxanthine‐guanine phosphoribosyl transferase levels were in some cases lower than the parental line but in three of the clonal lines were higher than the parental strain. The fact that azaguanine resistant lines constitute a biochemically heterogeneous population underscores the importance of careful characterization of mammalian cell culture variants.