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Relationship between cytoagglutination and saturation density of cell growth
Author(s) -
Weber Joseph
Publication year - 1973
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040810106
Subject(s) - saturation (graph theory) , agglutination (biology) , concanavalin a , hela , incubation , agglutinin , cell culture , microbiology and biotechnology , chemistry , biology , trypsin , cell , biochemistry , lectin , antibody , in vitro , immunology , enzyme , genetics , mathematics , combinatorics
The agglutination with concanavalin A and wheat germ agglutinin of the established malignant cells, HEp 2, KB, HeLa, TDB‐3, HTC and RV 3T3, and of the putatively normal cells, BHK 21, 3T3 and Wi‐38 was examined as a function of their saturation densities in culture. A positive correlation between the saturation density of the cell lines and the capacity to agglutinate was demonstrated. Incubation for 15 minutes with 1.25 mg/ml of trypsin converted non‐agglutinating and poorly agglutinating cells into agglutinable ones, while leaving the highly agglutinating lines largely unchanged. The magnitude of change in agglutination after trypsin treatment correlated inversely with saturation density. Although the extent of agglutination varied with the saturation density, the agglutinability of a particular line remained relatively unchanged at different cell densities.