Premium
A low molecular weight factor in lung‐conditioned medium stimulating granulocyte and monocyte colony formation in vitro
Author(s) -
Sheridan J. W.,
Metcalf D.
Publication year - 1973
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040810103
Subject(s) - colony stimulating factor , in vitro , haematopoiesis , agar , granulocyte , monocyte , bone marrow , chemistry , lung , granulocyte colony stimulating factor , granulocyte macrophage colony stimulating factor receptor , microbiology and biotechnology , biology , immunology , biochemistry , medicine , macrophage , macrophage colony stimulating factor , stem cell , bacteria , genetics , chemotherapy
Medium conditioned by excised whole lungs from endotoxin‐injected C 57 BL mice was highly active in stimulating hemopoietic colony formation, particularly of granulocytic type, in agar cultures of mouse bone marrow cells. The colony stimulating factor (CSF) in this material had an α 1 ‐α 2 electrophoretic mobility, was eluted from calcium phosphate gel by 0.04 M phosphate buffer and had an unusually low apparent S 20 W of 1.9. Sequestered polymor‐phonuclear neutrophils were excluded as a major source of this CSF. The high specific activity and ease of preparation of lung conditioned medium make it valuable both for the large scale production of CSF and as a source of an unusual type of CSF.