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Requirement for calcium ions in lymphocyte transformation stimulated by phytohemagglutinin
Author(s) -
Whitney Richard B.,
Sutherland Robert M.
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040800303
Subject(s) - calcium , nucleic acid , dna synthesis , chemistry , transformation (genetics) , magnesium , lymphocyte , dna , ion , biochemistry , microbiology and biotechnology , biology , immunology , gene , organic chemistry
Medium calcium ions were essential to the development of the lymphocyte transformation response induced by PHA. Magnesium ions could not substitute for calcium ions. Calcium exerted its influence during the induction phase of the process before DNA synthesis began since its removal after the response had fully developed did not alter subsequent levels of nucleic acid synthesis. The early development of RNA synthesis was prevented by low calcium levels indicating that calcium either directly influenced the initiation of this process or some prior event(s).