Premium
Transport changes in synchronously growing CHO and L cells
Author(s) -
Sander Greta,
Pardee Arthur B.
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040800214
Subject(s) - colcemid , isobutyric acid , cycloheximide , chinese hamster ovary cell , uridine , cell cycle , chemistry , microbiology and biotechnology , biology , mitosis , biochemistry , cell , cell culture , protein biosynthesis , genetics , rna , gene
A reduced rate of transport of 2‐amino‐isobutyric acid (AIB), was found to be associated with M and early G 1 periods in synchronized populations of CHO and L cells. A doubling of the transport rate occurred with progress of CHO cells further into G 1 , and the specific transport activity remained constant during the rest of the cycle, The rate of uridine and thymidine transport also doubled at this point in the cell cycle. There was no change in Km values. The rise in transport rate was blocked by cycloheximide, or when the cells were prevented from leaving metaphase by colcemid.