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A cell line which is temperature‐sensitive for cytokinesis
Author(s) -
Smith B. J.,
Wigglesworth N. M.
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040800212
Subject(s) - cytokinesis , mitosis , biology , cell culture , cell division , cell , microbiology and biotechnology , hamster , genetics
We have isolated several cell lines which are temperature‐sensitive for growth. One of these appears to be temperature‐sensitive for cytokinesis. It was isolated from a Syrian hamster cell line by selecting cells which were not killed by 1 μg/ml cytosine arabinoside at 39° but which grew normally at 31°. It shows an increased proportion of binucleate cells when shifted to the non‐permissive temperature and time‐lapse photomicroscopy shows that a high proportion of attempted mitoses fail at 39°, apparently at the stage of cytokinesis. The cells which have failed to complete mitosis reattach to the plate and have two normal‐size nuclei but otherwise behave normally.