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Effects of prednisolone on the distribution and activity of two phosphoesterases in membrane preparations from HeLa cells
Author(s) -
Costlow Mark E.,
Melnykovych George
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040800209
Subject(s) - hela , steroid , prednisolone , alkaline phosphatase , enzyme , membrane , cell culture , glucocorticoid , cell , cell membrane , biochemistry , enzyme assay , chemistry , in vitro , biology , microbiology and biotechnology , endocrinology , medicine , hormone , genetics
Abstract Activities of two membrane associated enzymes, viz. 5′‐nucleo‐tidase (EC 3.1.3.5) and alkaline phosphatase (EC 3.1.3.1) are known to be altered by glucocorticoid treatment of cells in culture. In certain HeLa cell lines, their activity is elevated, while in others, it is decreased. In order to confirm the data obtained previously with cell homogenates, cells were fractionated essentially as described by Bosmann, Hagopian and Eylar ('68) after 96 hours exposure to 0.5 μg/ml prednisolone. This treatment resulted in changes both in the specific activity and in the distribution of the two enzymes among the isolated membrane fractions. The alterations in specific activity in the individual membrane fractions reflected changes previously observed in preparations from whole cells. A shift in distribution of total enzymatic activity to fractions of higher density occurred in both cell lines in response to the steroid. These results may be indicative of cellular membrane alterations as a consequence of steroid treatment.