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Studies of calcium influx into squid giant axons with aequorin
Author(s) -
Hallett Mark,
Carbone Emilio
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040800208
Subject(s) - aequorin , luminescence , squid , calcium , bioluminescence , biophysics , action (physics) , superposition principle , chemistry , physics , biology , optoelectronics , biochemistry , quantum mechanics , organic chemistry , ecology
Calcium‐influx associated with the action potential was studied in squid giant axons using an EDTA‐free preparation of aequorin as a probe. Associated with an individual action potential there was a transient increase in aequorin luminescence and the time course of this increase was examined on a kinetic basis. The luminescent response associated with a train of action potentials was compared with that expected on the basis of superposition of individual responses. The analyses of luminescence curves produced by long trains of action potentials were complicated by failure of superposition. A long pulse of inward current was found to produce a very large enhancement of luminescence.