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Induction of cell division: Role of cell membrane sites
Author(s) -
Sivak Andrew
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040800203
Subject(s) - concanavalin a , wheat germ agglutinin , cell division , cell , proteolytic enzymes , biology , cell membrane , microbiology and biotechnology , cell culture , biochemistry , lectin , enzyme , in vitro , genetics
Density dependent inhibition of cell replication is released in stationary cultures of BALB/c‐3T3 fibroblasts by the potent tumor promoter for mouse skin, phorbol myristate acetate (PMA). The saturation density of these cultures, which is dependent on the serum concentration of the medium, is increased by PMA. Cell division, which can be induced by PMA or serum in stationary monolayers of BALB/c‐3T3 cells, is not blocked by several inhibitors of proteolytic enzymes or the plant lectins, concanavalin A or wheat germ agglutinin. Induction of cell replication by PMA or serum does not appear to be dependent on proteolytic activity, and the membrane sites associated with this induction appear to be distinct from the agglutinin‐binding sites.