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Ion transport by ouabain resistant and sensitive ehrlich ascites carcinoma cells
Author(s) -
Mayhew E.
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040790314
Subject(s) - ouabain , uridine , strophanthin , digitoxin , cell culture , chemistry , ascites , ehrlich ascites carcinoma , biology , cell , medicine , biophysics , endocrinology , microbiology and biotechnology , biochemistry , digoxin , in vitro , sodium , rna , heart failure , genetics , organic chemistry , gene
Cell division, net Na + ‐K + and amino‐acid transport of cultured Ehrlich ascites is reversibly inhibited by Ouabain at a final concentration of 1 × 10 –3 M. A line of Ehrlich ascites cells resistant to the growth inhibiting effects of Ouabain has been developed. These cells behave similarly to Ouabain‐sensitive cells in the following respects doubling time, S phase time, chromosome number, cell surface charge density, rate of incorporation of C 14 Uridine and 3 H‐Thymidine, sensitivity to Digoxin and Digitoxin, steady state Na + , K + levels and rate of loss of K + and gain of Na + in cold. Ouabain resistant cells differ from sensitive cells only with respect to the effect of ouabain on active Na + , K + transport. Although Ouabain inhibits active Na + , K + transport in sensitive cells it has no significant effect in resistant cells.