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Density dependent regulation of growth in suspension cultures of L‐929 cells
Author(s) -
Glinos André D.,
Werrlein Robert J.
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040790109
Subject(s) - intracellular , mitosis , cell culture , cell division , centrifugation , cell growth , biology , cell , microbiology and biotechnology , differential centrifugation , suspension (topology) , dilution , suspension culture , steady state (chemistry) , biochemistry , chemistry , genetics , physics , mathematics , homotopy , pure mathematics , thermodynamics
Suspension cultures of L‐929 fibroblasts grown to densities of 6 to 10 × 10 6 cells/ml through daily centrifugation and resuspension in fresh media, have been maintained for periods up to five months without change in viability or cell size. DNA synthesis and mitosis in these cultures is limited to 5% of the cells per day, a fraction very nearly equal to the fraction of cells rendered nonviable, most likely during the manipulations associated with medium renewal. The kinetics of the flow of cells into the S and M periods following (a) renewal of the medium and (b) dilution of the high density cultures, suggest that the large majority of the cells are in a G 0 or early G 1 phase, resuming growth readily in response to decreased cell density. This is further indicated by the sequence of the marked shifts occurring in the cell volume distribution spectrum of the high density cultures after dilution. Long term, steady state regulation of growth with retention of intact viability was thus demonstrated in the case of a long established aneuploid cell line. The fact that this occurs in suspension but not in attached cultures, supports the concept that impairment of growth control in such cells affects predominantly regulatory mechanisms located at the cell surface rather than those concerned with intracellular synthesis and metabolism.