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Phosphate transport in ehrlich ascites tumor cells and the effect of arsenate
Author(s) -
Levinson Charles
Publication year - 1972
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040790108
Subject(s) - arsenate , pi , phosphate , extracellular , michaelis–menten kinetics , kinetics , chemistry , ascites , ehrlich ascites , tumor cells , membrane transport , extracellular fluid , biochemistry , membrane , biophysics , biology , enzyme , arsenic , medicine , cancer research , enzyme assay , physics , organic chemistry , quantum mechanics
The effect of extracellular Pi and arsenate on Pi‐transport in Ehrlich ascites tumor cells has been studied. Pi‐transport can be described by Michaelis‐Menten kinetics; the maximal flux equal to 44 mmoles (kg cell water) −1 hour −1 and K m equal to 3.3 × 10 −4 M . Arsenate is a competitive inhibitor of Pi‐transport with an inhibition constant (K i ) equal to 2.41 × 10 −3 M . The data support the hypothesis that cellular Pi is regulated by the cell membrane through the mediation of a carrier system.