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Adherence column and buoyant density separation of bone marrow stem cells and more differentiated cells
Author(s) -
Metcalf D.,
Moore M. A. S.,
Shortman K.
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040780313
Subject(s) - in vitro , bone marrow , density gradient , spleen , in vivo , chromatography , agar , column chromatography , agar gel , microbiology and biotechnology , chemistry , biology , immunology , biochemistry , genetics , bacteria , physics , quantum mechanics
Mouse bone marrow cells were separated by adherence column and albumin density gradient procedures, assaying for spleen colony forming units ( in vivo CFU's), agar colony forming cells ( in vitro CFC's) and cluster forming cells. Column filtrates were enriched for CFU's whereas in vitro CFC's and cluster‐forming cells were enriched in adherent fractions. Gradient separation of these column fractions gave density distribution profiles indicating the non‐identity and heterogeneity of CFU's and in vitro CFC's.