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Cultures from adult rat liver cells I. Establishment of monolayer cell‐cultures from normal liver
Author(s) -
Iype P. T.
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040780217
Subject(s) - collagenase , plating efficiency , doubling time , hyaluronidase , cell culture , biology , monolayer , in vitro , cell , tissue culture , microbiology and biotechnology , biochemistry , enzyme , genetics
Establishment of monolayer cell‐cultures from adult normal rat liver is described using cells isolated by a collagenase‐hyaluronidase perfusion technique. The cells plated originally were polygonal in shape and contained a large number of mitochondria. However, cells with less granules were also observed in the primary culture, after a few days of plating. In subsequent subcultures, the latter type of cells could be grown continuously. After an initial lag, the cells attained a doubling time of 55 hours and a plating efficiency of 20%. The cells are “normal” with respect to their karyology, morphology and growth pattern. Difficulties encountered during the culture and the possible uses of these cultured cells in studies on carcinogenesis in vitro are discussed.