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Effect of 5‐bromodeoxyuridine on hyaluronic acid synthesis of a clonal hybrid line of mouse and Chinese hamster in culture
Author(s) -
Koyama Hideki,
Ono Tetsuo
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040780214
Subject(s) - chinese hamster , bromodeoxyuridine , dna synthesis , cell culture , thymidine , cell growth , biology , hamster , cell division , chinese hamster ovary cell , inhibitory postsynaptic potential , biochemistry , hyaluronic acid , microbiology and biotechnology , cell , in vitro , genetics , endocrinology
A clonal hybrid line, B‐6, derived from the fusion of mouse and Chinese hamster cell lines, has an inheritable ability to synthesize and secrete hyaluronic acid (HA). In order to elucidate the control mechanism of expression of this differentiated function, we studied the effect of 5‐bromodeoxyuridine (BUDR), a thymidine (TDR) analog on the growth and HA synthesis of this hybrid line. The presence of BUDR at 0.5–1 μg/ml of medium had no effect on cell multiplication, but reduced HA production by approximately 25 to 40%. Treatment with 5 to 25 μg/ml of the analog resulted in about 15% decrease of the total cell growth, or 30% decrease of the viable cell growth, while mucopoly‐saccharide production was reduced by more than 70%. These results indicate that BUDR affects HA synthesis more strongly than it does cell growth. Inhibition was found to be detectable after two days of culture with BUDR. This effect was prevented by the simultaneous addition of TDR in a concentration more than twice that of the analog. Furthermore the inhibitory effect was reversible, since a gradual but complete recovery of HA synthesis occurred after removal of BUDR from the medium. In contrast, the growth rate of B‐6 cells was discovered to be more sensitive than HA synthesis to the inhibitory action of either excess TDR or another analog, 5‐fluorodeoxyuridine (FUDR).