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Phosphorus incorporation in the ehrlich ascites tumor cell
Author(s) -
Levinson Charles,
Gordon Edward D.
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040780213
Subject(s) - labelling , adenylate kinase , phosphate , oxidative phosphorylation , biochemistry , kinetics , ascites , metabolism , phosphorylation , flux (metallurgy) , chemistry , phosphorus , tumor cells , ehrlich ascites , biology , enzyme , cancer research , medicine , physics , organic chemistry , quantum mechanics
Data from isotopic uptake experiments were used to measure the kinetics of labelling of cellular phosphate, ATP and ADP in the Ehrlich ascites tumor cell. The results show that steady state phosphate exchange flux was 0.333 ± 0.052 (S.E.) μmoles per 10 7 cells per hour at 37°, and that the specific activity of phosphate was the same as P γ ATP. Metabolic inhibition reduced the phosphate flux by 30–50%. A model, based on oxidative phosphorylation and the adenylate kinase reaction is used to interpret the labelling sequence of P β ATP and P β ADP, and its dependence on P γ ATP.