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Surface charge and cell volume of synchronized mouse lymphoblasts (L5178‐Y)
Author(s) -
Shank Brenda Buckhold,
Burki H. John
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040780211
Subject(s) - mitosis , lymphoblast , cell , microbiology and biotechnology , biophysics , cell division , cell cycle , biology , chemistry , cell culture , biochemistry , genetics
Cellular electrophoretic mobility, and therefore, surface charge/ unit area, was found to be constant throughout the cell cycle of synchronized L5178‐Y mouse lymphoblasts, remaining at – 1.21 μ sec −1 V −1 cm. Measured cell volumes of these synchronized cells increased linearly over the cell cycle and at mitosis each cell divided into two cells, each cell having half of the parent volume. An hypothesis is presented which explains the presence of a mobility peak at mitosis in cells which normally are grown attaching to a glass surface on the basis of differential morphological changes during mitosis; cells such as the L5178‐Ys, which do not attach to glass normally, do not undergo such morphological changes at mitosis and, therefore, would not demonstrate a mobility peak at that time.

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