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Innervation and acetylcholine sensitivity of skeletal muscle cells differentiated in vitro from chick embryo
Author(s) -
Kano Masaakira,
Shimada Yutaka
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040780210
Subject(s) - acetylcholine , myocyte , anatomy , neuromuscular transmission , in vitro , biology , neuromuscular junction , stimulation , electrophysiology , spinal cord , embryo , microbiology and biotechnology , neuroscience , endocrinology , biochemistry
Trypsin‐dissociated myoblasts from leg muscle of 12‐day chick embryos have been cultured in monolayers. After four days the muscle cultures have been confronted with fragments of the spinal cord of six‐day chick embryos. Electrophysiological and morphological analysis demonstrate that characteristic neuromuscular transmission can develop in these cultures. Electrical stimulation of the cord fragment evokes contractions of innervated muscle fibers, from which end plate potentials and miniature end plate potentials with average frequency around one per second or more can be recorded. D‐tubocurarine (1 μg/ml) suppresses reversibly these synaptic potentials. Non‐innervated muscle fibers are sensitive to acetylcholine over all their surface, while innervated muscle fibers are sensitive at the regions where structures suggestive of motor end plate (“bulb‐type”) are found. We can conclude that neuromuscular connections developed in vitro in our experiments are functional in respect of transmission of impulses but also in respect of neurotrophic influences for restriction of chemosensitivity.