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Modification of cell surface antigenicity as a function of culture conditions
Author(s) -
Pfeiffer S. E.,
Herschman H. R.,
Lightbody J. E.,
Sato G.,
Levine L.
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040780118
Subject(s) - antigenicity , antigen , microbiology and biotechnology , biology , cell culture , in vitro , function (biology) , cell , autocrine signalling , immunology , biochemistry , genetics
Adaptation of monolayer cultures of a clonal line of rat glial cells to suspension culture resulted in the nearly complete loss of certain surface antigens. This change in surface antigenicity was paralleled by the loss of the ability of the cells to accumulate in vitro a protein specific to the nervous system (“S100‐protein”). In contrast, when glial cells were co‐cultivated in monolayer culture with another cell line apparently lacking these surface antigens, the number of these antigens was markedly increased. The possibility of a causal relationship between the changes in the surface antigenicity and the expression of differentiated function is considered.