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Photo‐reversal of the circadian rhythm in the proliferative activity of the mouse small intestine
Author(s) -
Sigdestad Curtis P.,
Lesher S.
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040780115
Subject(s) - circadian rhythm , crypt , thymidine , mitosis , biology , rhythm , medicine , endocrinology , andrology , dna , microbiology and biotechnology , biochemistry
Mice (C57Bl) were placed for 35 days in a room with reversed light cycle; lights came on at 1800 hours and off at 0600 hours. At six hour intervals throughout the day three mice were injected with tritiated thymidine and sacrificed 30 minutes later. Crypts were dissected for radioautography and determination of disintegrations per minute per crypt, the number of cells in mitosis and the number of cells synthesizing DNA was determined. The results reported clearly demonstrate reversal of the circadian rhythm in the parameters tested.