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Energy transfer in a bioluminescent system
Author(s) -
Morin James G.,
Hastings J. W.
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040770305
Subject(s) - bioluminescence , photoprotein , fluorescence , excited state , energy transfer , luminescence , biophysics , green fluorescent protein , chemistry , molecule , photochemistry , biochemistry , biology , materials science , optoelectronics , physics , chemical physics , optics , atomic physics , organic chemistry , gene
Many (but not all) of the bioluminescent systems in coelenter‐ates involve energy transfer from an excited product molecule of the calcium activated photoprotein to a second species, the green fluorescent protein, with emission at 508 nm from its excited state. Although all the luminescent coelen‐terates studied possess photoproteins, not all of them have the green fluorescent protein. This green fluorescent molecule is localized in the luminescent cells; they can thus be easily distinguished by fluorescence microscopy. The active components occur in subcellular particles; these have been isolated in an active form by homogenization in isotonic (to sea water) salt solutions.