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Cell surface negativity and the binding of positively charged particles
Author(s) -
Weiss L.,
Zeigel R.
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040770208
Subject(s) - neuraminidase , negativity effect , staining , ferric , biophysics , incubation , electrophoresis , negative stain , ribonuclease , chemistry , cell , biochemistry , biology , electron microscope , enzyme , genetics , inorganic chemistry , psychology , rna , social psychology , physics , gene , optics
The binding of positively charged, colloidal ferric oxide particles to the surfaces of Ehrlich ascites tumour cells, before and after incubation with neuraminidase and/or ribonuclease, has been studied by electron microscopy. An attempt has been made to correlate the amount of binding observed, with the electrophoretic mobilities of the cells under similar conditions to those under which they were exposed to the ferric oxide. Although a progressive loss of staining was observed with progressive loss of cellular net surface negativity, neither property was predictable from knowledge of the other. Some of the difficulties inherent in quantitative staining techniques of this type are discussed.