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Stimulation by epinephrine of adenyl cyclase activity, cyclic AMP formation, DNA synthesis and cell proliferation in populations of rat thymic lymphocytes
Author(s) -
MacManus J. P.,
Whitfield J. F.,
Youdale T.
Publication year - 1971
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040770112
Subject(s) - dna synthesis , thymocyte , epinephrine , cyclase , stimulation , caffeine , medicine , endocrinology , biology , dna , hormone , in vitro , cell division , nucleotide , population , cyclic nucleotide , enzyme , mitosis , cell growth , cell , biochemistry , microbiology and biotechnology , t cell , immune system , immunology , environmental health , gene
During the first ten minutes after the beginning of a continuous exposure of rat thymocyte populations (maintained in vitro ) to epinephrine, there is an increase in the cellular concentration of cyclic AMP. The hormone also increases the activity of a crude preparation of the thymocyte's cyclic AMP‐forming enzyme, adenyl cyclase. Between 30 and 45 minutes after the beginning of exposure to epinephrine, an additional part of the cell population begins to synthesize deoxyribonucleic acid (DNA). These changes are finally followed two to four hours later by an increase of the flow of cells into mitosis. Since cyclic AMP itself is known to stimulate both the initiation of DNA synthesis and thymocyte proliferation, and since the mitogenic action of epinephrine is shown to be potentiated by caffeine and inhibited by imidazole, it is concluded that the mitogenic action of this hormone is mediated by the cyclic nucleotide.