Premium
Variations in the synthesis and assembly of immunoglobulins by mouse myeloma cells: A genetic and biochemical analysis
Author(s) -
Scharff M. D.,
Bargellesi A.,
Baumal R.,
Buxbaum J.,
Coffino P.,
Laskov R.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040760311
Subject(s) - antibody , immunoglobulin light chain , secretion , gamma globulin , multiple myeloma , kinetics , plasma cell , globulin , microbiology and biotechnology , biology , chemistry , biochemistry , immunology , physics , quantum mechanics
The synthesis, assembly, and secretion of gamma globulin has been examined using cells from a mouse plasma cell tumor. The cells were then adapted to continuous culture and individual clones were obtained which differed from each other in the relative amounts of heavy and light chains synthesized, in the absolute amounts of gamma globulin being produced, and in the details of the assembly process. Finally, the kinetics, pathway, and completeness of assembly were examined in a variety of tumors and variants of these tumors.