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Selection of synchronous Chlorella cells by centrifugation to equilibrium in aqueous ficoll
Author(s) -
Hopkins H. A.,
Sitz T. O.,
Schmidt R. R.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040760214
Subject(s) - chlorella pyrenoidosa , ficoll , centrifugation , chlorella , aqueous solution , differential centrifugation , selection (genetic algorithm) , biology , chemistry , chromatography , biological system , algae , biochemistry , in vitro , botany , computer science , peripheral blood mononuclear cell , artificial intelligence
Synchronous daughter cells of Chlorella pyrenoidosa can be selected, on the basis of their unique density, from cultures growing in continuous light by centrifugation to equilibrium in aqueous Ficoll. The degree of synchrony, as judged from cell release data, is equivalent to or better than that obtained by intermittent‐illumination procedures.