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Mammalian cell fusion III. A hela cell inducer of premature chromosome condensation active in cells from a variety of animal species
Author(s) -
Johnson R. T.,
Rao P. N.,
Hughes H. D.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040760204
Subject(s) - premature chromosome condensation , chinese hamster ovary cell , hela , biology , mitosis , heterologous , cell fusion , microbiology and biotechnology , cell , xenopus , baby hamster kidney cell , cell type , chromosome , embryonic stem cell , embryo , cell culture , inducer , hamster , genetics , cell cycle , gene
The phenomenon of premature chromosome condensation (PCC) is induced in unstimulated horse lymphocytes, bovine spermatozoa, Chinese hamster ovary cells, embryonic chick fibroblasts and erythrocytes, Xenopus kidney and mosquito cells by fusing each of these cell types with HeLa cells blocked in mitosis. Thus it becomes possible to visualize chromosomes even from non‐multiplying cells of heterologous species, such as, chick erythrocytes and bovine spermatozoa.