Premium
A new method of cloning mammalian tissue culture cells in suspension
Author(s) -
Walters R. A.,
Hutson J. Y.,
Burchill B. R.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040760112
Subject(s) - cloning (programming) , chinese hamster , suspension (topology) , chinese hamster ovary cell , suspension culture , plating (geology) , cell culture , microbiology and biotechnology , tissue culture , chromatography , chemistry , biology , biochemistry , genetics , in vitro , mathematics , computer science , paleontology , homotopy , pure mathematics , programming language
Chinese hamster cells (line CHO) were cloned in suspension with a carboxymethylcellulose‐containing medium, and the colonies were counted with an electronic particle counter after enzymatically degrading the carboxymethylcellulose with cellulase. Survival of x‐irradiated cells was determined by both the standard plating technique and the suspension technique, and comparable results were obtained.