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Beneficial effects of embryo extract on cultured rabbit cartilage cells
Author(s) -
Ham Richard G.,
Murray Louann W.,
Sattler Gerald L.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040750311
Subject(s) - embryo , sephadex , cartilage , fetus , fetal bovine serum , chemistry , rabbit (cipher) , microbiology and biotechnology , andrology , biology , biochemistry , in vitro , anatomy , pregnancy , enzyme , medicine , genetics , statistics , mathematics
Growth and the expression of cartilage‐like differentiation by adult rabbit chondrocytes is stimulated greatly by the Sephadex G‐25 macromolecular fraction of chicken embryo extract (CEEM). A small amount of fetal bovine serum or the macromolecular fraction of fetal bovine serum (FBSM) is also needed for growth. Medium F12 supplemented with CEEM plus FBSM supports moderate clonal growth of the rabbit chondrocytes with excellent expression of differentiated properties. The effects of CEEM on growth and the expression of differentiation have been quantitated by measurement of radioactive sulfate incorporation and total protein synthesis.