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Induction of fatty acid synthesis in cultured mammalian cells: Effects of cycloheximide and x‐rays
Author(s) -
Raff R. A.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040750310
Subject(s) - cycloheximide , fatty acid synthesis , protein biosynthesis , enzyme , fatty acid , biochemistry , biosynthesis , chemistry , biology
It was found that L cells deprived of exogenous fatty acids responded adaptively by increased synthesis of fatty acids. This adaptive increase was found to be sensitive to cycloheximide presented at the time of removal of exogenous lipids, but was not sensitive to cycloheximide added six hours after removal of lipids. It was concluded that the adaptive increase in fatty acid synthesis represents a case of enzyme induction. The effect of x‐rays on this process was studied. It was found that induction was inhibited to a greater extent than total protein synthesis. Factors of potential importance to such an effect are discussed.