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Evidence for the existence of multipotential lympho‐hematopoietic stem cells in the adult rat
Author(s) -
Nowell Peter C.,
Hirsch Bruce E.,
Fox Dianne H.,
Wilson Darcy B.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040750203
Subject(s) - biology , haematopoiesis , clone (java method) , stem cell , spleen , bone marrow , histocompatibility , immunology , homologous chromosome , major histocompatibility complex , lymphocyte , microbiology and biotechnology , antigen , genetics , gene , human leukocyte antigen
Rats were given near‐lethal doses of x‐ray to produce clones of hemic cells marked by radiation‐induced chromosome abnormalities. Subsequently, bone marrow from these rats was injected into lethally irradiated mice to form erythropoietic spleen colonies; and peripheral blood lymphocytes from the same rats were stimulated to proliferate in a mixed lymphocyte interaction (MLI), an immunological response to histocompatibility isoantigens. Chromosome markers indicated that in several instances the cells of an erythroid spleen colony and a proportion of the lymphocytes reacting in the MLI were progeny of the same stem cell in the donor rat. In addition, lymphocytes of the same radiation‐marked clone were shown to proliferate in response to several different histocompatibility isoantigens. The data indicate the presence in the adult rat of a primitive lymphohematopoietic stem cell capable of yielding both erythroid and lymphoid progeny. The findings also suggest that immunological specificity is determined during lymphoid differentiation, subsequent to the stem cell stage.