Premium
Nuclear alterations in mammalian cells induced by L‐canavanine
Author(s) -
Hare J. D.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040750115
Subject(s) - canavanine , dna synthesis , nuclear membrane , dna replication , dna , microbiology and biotechnology , nuclear protein , nuclear dna , cell nucleus , biology , arginine , chemistry , cell , biochemistry , nucleus , gene , amino acid , transcription factor , mitochondrial dna
Mammalian cells treated four hours with the L‐arginine analogue, L‐canavanine (Cana), develop alterations in nuclear architecture characterized by the formation of irregular aggregates of nucleoplasmic contents, frequently attached to the nuclear membrane. On the basis of previous studies showing that Cana is incorporated into nuclear proteins and inhibits DNA synthesis over similar periods of exposure, it is postulated that the formation of canavanyl protein‐DNA aggregates interfere with DNA replication.