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Control of multiplication of uninfected rat cells and rat cells converted by murine sarcoma virus
Author(s) -
Temin Howard M.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040750113
Subject(s) - multiplication (music) , dna synthesis , cell division , stimulation , biology , cell culture , mitosis , microbiology and biotechnology , embryo , tissue culture , cell , dna , in vitro , biochemistry , genetics , endocrinology , physics , acoustics
The rate of multiplication of rat embryo fibroblasts in monolayer culture depends upon the amount of multiplication‐stimulating activity in the culture medium, as well as the efficiency of stimulation by and utilization of this activity. Multiplication‐stimulating activity is defined by its capacity to stimulate DNA synthesis and cell division in stationary populations of cells. Usually, multiplication stimulating activity is supplied as serum in cell culture media, but rat cells also produce it. A comparison of multiplication of uninfected and Murine Sarcoma virus‐converted rat cells showed that converted cells multiplied at a greater rate than did uninfected cells, with the use of less or the same amount of multiplication‐stimulating activity; the converted cells produced cells produced an inhibitor of multiplication‐stimulating activity, and the efficiency of stimulation of DNA synthesis was similar for uninfected and converted cells. It appears that in the presence of serum the efficiency of utilization of multiplication‐stimulating activity is greater for converted cells than for uninfected rat cells.