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Characterization of RNA molecules restricted to the nucleus in mouse L‐cells
Author(s) -
Shearer Ruth W.,
McCarthy Brian J.
Publication year - 1970
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040750112
Subject(s) - rna , cytoplasm , nucleus , cell nucleus , dna , small nuclear rna , biology , microbiology and biotechnology , messenger rna , cell , cell fractionation , gene , non coding rna , chemistry , biochemistry , enzyme
Abstract RNA molecules which are restricted to the nucleus in mouse L‐cells were characterized by the technique of RNA/DNA hybridization. Competition of cytoplasmic RNA with labeled nuclear RNA of various sizes revealed that the RNA restricted to the cell nucleus is heterogeneous in size. Competition for sites on fractions of mouse DNA of various base compositions indicated that this unstable RNA is also heterogeneous in base composition. Fractionation of nuclei into three subfractions failed to separate the uniquely nuclear RNA from the precursors of cytoplasmic RNA. The significance of the selective transport of RNA from the nucleus to the cytoplasm and its importance in the control of gene activity in eucaryotic cells is discussed.