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Studies on colony formation in vitro by mouse bone marrow cells . I. Continuous cluster formation and relation of clusters to colonies
Author(s) -
Metcalf Donald
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040740313
Subject(s) - incubation , incubation period , in vitro , bone marrow , biology , macrophage , cluster (spacecraft) , period (music) , stimulation , microbiology and biotechnology , immunology , genetics , biochemistry , endocrinology , physics , computer science , acoustics , programming language
Colony formation in vitro by mouse bone marrow cells following stimulation by human urine was analysed over a 7‐day incubation period. There was a linear increase with time in the number of cell aggregates (clusters) developing in such plates. Early in the incubation period all clusters were granulocytic although later macrophage clusters developed. Although most fully developed colonies were composed of macrophages, mapping and transfer studies showed that at least half of these had initially arisen early in the incubation period as granulocytic clusters.