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Hemoglobin and ferritin synthesis in erythroid cells in prolonged marrow cell cultures
Author(s) -
Gabuzda Thomas G.,
Silver Ruth K.,
Melvin Mary,
Windus Constance,
Sachs Carl
Publication year - 1969
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040740307
Subject(s) - cytoplasm , hemoglobin , heme , biology , ferritin , globin , cell culture , microbiology and biotechnology , tissue culture , suspension culture , cell , biochemistry , genetics , in vitro , enzyme
A method is described which maintains viable erythroid cells in tissue culture for periods from nine to twenty days. These cells appear predominantly as small round cells with scanty cytoplasm. They synthesize both heme and globin and are relatively more numerous free in suspension than in the adherent monolayer. Ferritin isomorph may serve as a convenient marker in tissue culture of cells of erythroid origin, suggesting that such cells may persist despite a completely transformed appearance and a loss of the ability to produce hemoglobin.